Ashby’s Mannitol Broth

Principle Ashbys mannitol broth is described by Subba Rao (1977) for the isolation and cultivation of a non-symbiotic nitrogen-fixing bacteria, Azotobacter. Ashby’s media is composed of mannitol, dipotassium phosphate, magnesium sulfate, sodium chloride, potassium sulfate, and calcium carbonate. Mannitol provides carbon sources and atmospheric nitrogen serves nitrogen sources. Dipotassium Phosphate is a buffering agent. The other salts provide various essential ions required for promoting the growth of Azotobacter. The presence of calcium carbonate stimulates growth and increases the availability of phosphorus, sulfur, and magnesium to the organisms. Use: Recommended for the cultivation of mannitol fermenting Azotobacter from soil samples. PARAMETERS STANDARD VALUES PHYSICAL PARAMETERS: Dehydrated Powder A fine, free-flowing, hygroscopic powder. Color Off-white colored powder. Solution Solubility Soluble in Distilled / de-ionized water at 50˚ C Color Milky white colored solution with stealing at the bottom on standing pH 7.4 ±0.2 at 25 ˚C (25.70 g/ l) ,Intend use : • 18% GST extra on Product • Packaging, Handling & Transport charges (with GST) extra as applicable. • Delivery period : 3-4 weeks or earlier as per availability.