Antibiotic Assay Medium 32

Principle The media is composed according to the USP, recommended as an antibiotic assay medium. It is composed of peptone, casein peptone, yeast extract, meat extract (equivalent to beef extract), dextrose, manganese sulphate and agar. Peptone, casein peptone, yeast extract and meat peptone provide nitrogen, carbon, long-chain amino acids, vitamins and essential nutrients for the growth of microorganisms. Dextrose serves as an energy source. Manganese sulphate in this medium facilitates the sporulation and growth of Bacillus subtilis. Agar is the solidifying agent. Use: Recommended for microbiological assay of antibiotics (Dihydrostreptomycin and Vancomycin) against variety of microbes especially Bacillus subtilis in accordance with United States Pharmacopoeia. PARAMETERS STANDARD VALUES PHYSICAL PARAMETERS: Dehydrated Powder A fine, free flowing, hygroscopic powder. Color Light beige colored powder. Solution Solubility Soluble in Distilled / de-ionized water at 50˚ C Color Light amber colored opalescent pH 6.6 ±0.1 at 25 ˚C (30.80 g/ l) Agar Plates Solidification 40 ˚C Color Light amber colored opalescent gel ,Intend use : • 18% GST extra on Product • Packaging, Handling & Transport charges (with GST) extra as applicable. • Delivery period : 3-4 weeks or earlier as per availability.